Lambda DNA (N6-methyladenine-free) from New England Biolabs

Product Specs
ItemLambda DNA (N6-methyladenine-free)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberN3013S
Source/Expression SystemIsolated from bacteriophage lambda (cI857ind 1 Sam 7)
Molecule NameUnmethylated DNA
Quantity250 µg
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Ladder TypeLambda DNA
Size Range48.5 Kbp
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Visualizing carboxyl-terminal domain of RNA polymerase II recruitment by FET fusion protein condensates with DNA curtainsBiophysics ReportsApril 30, 2022Zuo Linyu, Center for Quantitative Biology and Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China, Ding Jiawei, Qi Zhi1 ) Microsatellite DNA containing 25× EWS-FLI1 target sites is inserted into Lambda DNA (NEB, Cat. N3013S) XhoI/NheI sites. The ligation product is packaged into MaxPlax™ Lambda Packaging Extracts (Epicentre,

(2) mTORC1 stimulates cell growth through SAM synthesis and m⁶A mRNA-dependent control of protein synthesisMolecular cellMay 20, 2021Villa E, Sahu U, O'Hara BP, Ali ES, Helmin KA, Asara JM, Gao P, Singer BD, Ben-Sahra I.estimated by the measured frequency of unmethylated CpGs in λ-bacteriophage DNA (New England BioLabs, #N3013S) added at a 1:200 mass ratio to each sample. We then prepared libraries using the Pico Methyl-Seq Library Prep Kit

(3) Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasionCellApril 29, 2021Gangoso E, Southgate B, Bradley L, Rus S, Galvez-Cancino F, McGivern N, Güç E, Kapourani CA, Byron A, Ferguson KM, et al.Methyl-Seq System 1- 16 (Tecan Genomics Inc, #0353). 0.5ng of unmethylated λDNA (New England Biolabs, #N3013S) was spiked into each DNA sample to allow assessment of the bisulfite-conversion reaction efficiency.

(4) The lung microenvironment shapes a dysfunctional response of alveolar macrophages in agingJournal of Clinical InvestigationFebruary 15, 2021Alexandra C. McQuattie-Pimentel et al.conversion efficiency was 99.6% as estimated by the measured percentage of unmethylated CpGs in λ-bacteriophage DNA (N3013S, New England BioLabs) spiked into each sample. Equimolar ratios of 4 libraries per run were sequenced

(5) DNA methylation and gene expression signatures are associated with ataxia-telangiectasia phenotypeScientific ReportsDecember 1, 2020Sharon A. McGrath-Morrow, Roland Ndeh, Kathryn A. Helmin, Basil Khuder, Cynthia Rothblum-Oviatt, Joseph M. Collaco, Jennifer Wright, Paul A. Reyfman, Howard M. Lederman, Benjamin D. Singerestimated by the measured percent of unmethylated CpGs in λ-bacteriophage DNA (New England BioLabs N3013S) added at a 1:200 mass ratio to each sample. In total, we determined the methylation status of 6,697,115

(6) Maintenance DNA methylation is essential for regulatory T cell development and stability of suppressive functionJournal of Clinical InvestigationNovember 9, 2020Kathryn A. Helmin et al.of Clinical Investigation unmethylated CpGs in λ-bacteriophage DNA (New England BioLabs, catalog N3013S) added at a 1:200 mass ratio to each sample. Processing and analysis were conducted as previously described

(7) Uncoupling histone H3K4 trimethylation from developmental gene expression via an equilibrium of COMPASS, Polycomb, and DNA methylationNature GeneticsJune 1, 2020Delphine Douillet et al.methylation frequency of an unmethylated λ-bacteriophage spike-in control genome (New England BioLabs, N3013S). Libraries were prepared with the Pico Methyl-Seq Library Prep Kit (Zymo Research) using Illumina TruSeq

(8) The gene silencing protein MORC-1 topologically entraps DNA and forms multimeric assemblies to cause DNA compaction.Molecular CellAugust 22, 2019HyeongJun Kim et al.aaa aaa aaa aaa/Digoxigenin/-3’) was annealed to each cos site of genomic bacteriophage λ-DNA (NEB N3013S). The biotin at one end of the substrate was used for surface-tethering of the DNA to a neutravidin

(9) Mitochondrial complex III is essential for suppressive function of regulatory T cellsNatureJanuary 1, 2019Samuel E. Weinberg et al.conversion at CpG motifs in un-methylated λ-bacteriophage DNA spike-in controls (New England BioLabs N3013S). We then used the Pico Methyl-Seq Library Prep Kit (Zymo Research) to create indexed Illumina-compatible

(10) Droplet barcoding for massively parallel single-molecule deep sequencingNature CommunicationsSeptember 1, 2016Freeman Lan, John R. Haliburton, Aaron Yuan, Adam R. Abateseven known templates, eight DNA templates are amplified from 5 ng of phage lambda genomic DNA (NEB: N3013S) using 500 nM of primer sets (see oligonucleotides listed in Supplementary Table 1) using 1 × NEB phusion

(11) De novo sequencing and variant calling with nanopores using PoreSeqNature BiotechnologyOctober 1, 2015Tamas Szalay, Jene A. GolovchenkoDNA fragment sizes of approximately 8kbp. One microgram of Lambda DNA (New England Biolabs, cat. no. N3013S) in a total volume of 80 microliters water was applied to a Covaris g-TUBE and centrifuged, according

(12) Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopyNature ProtocolsMarch 1, 2013Anthony L. Forget, Christopher C. Dombrowski, Ichiro Amitani, Stephen C. Kowalczykowskibuffer #2 (NEB buffer supplied with Klenow Fragment DNA polymerase I NEB #B7002S) Phage λ DNA (NEB #N3013S) Potassium hydroxide (KOH Sigma-Aldrich #221473-1KG) CAUTION corrosive; handle wearing goggles, lab

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